item Absorbance Subtraction Amplitude Modulation Extended Ratio Subtraction Chemometric Reported** method MOX HCT Drug MOX HCT MOX HCT MOX HCT PLS PCR PLS PCR MOX HCT Mean 99.98 100.20 99.92 100.09 100.10 99.91 100.46 100.43 100.33 100.34 99.37 100.83 S.D 0.526 0.563 0.519 0.529 0.706 0.670 0.722 0.805 0.729 0.666 1.245 1.321 R.S.D% 0.526 0.562 0.519 0.529 0.705 0.671 0.719 0.802 0.727 0.664 1.253 1.310 n 5 5 5 5 5 5 8 8 8 8 5 5 Variance 0.277 0.317 0.269 0.280 0.498 0.449 0.521 0.648 0.531 0.444 1.550 1.745 Student´s 1.01 0.981 0.912 1.164 1.141 1.390 1.781 1.696 0.776 0.770     t-test * (2.306)* (2.306) (2.306) (2.306) (2.306) (2.306) (2.201) (2.201) (2.201) (2.201) - - F-value* 5.596 5.505 5.762 6.232 3.112 3.886 2.975 2.392 3.286 3.930       (6.39)* (6.39) (6.39) (6.39) (6.39) (6.39 (6.09)* (6.09) (6.09) (6.09) - - *Average of three determinations ** Corresponding to their ratio in pharmaceutical formulation

Table 7: Results obtained for the analysis of binary mixtures of MOX and (validation set) by the multivariate spectral analysis methods.