Experimental parameter Number of theoretical plates Capacity factor K`
LAM KCZ IDV LAM KCZ IDV
pH 3.0 2360 2880 4310 4.40 7.76 9.0
4.0 2410 3150 4470 4.41 7.75 9.0
4.5 2470 3240 4580 4.43 7.71 8.94
5.0 2435 3210 4450 4.38 7.60 8.80
6 2360 2910 4360 4.38 7.60 8.90
7.0 2310 2650 4280 4.40 7.58 8.90
SDS Conc. 0.05 2340 3170 4450 5.0 8.3 9.7
0.07 2470 3240 4580 4.43 7.71 8.94
0.1 2440 3120 4420 3.85 7.3 8.3
0.12 2350 2920 4320 3.0 6.4 7.6
0.15 2240 2840 4110 2.6 6.0 7.0
Type of organic
modifier
Methanol 1180 1850 2280 4.6 8.0 9.5
Ethanol 2250 2650 3110 4.5 7.9 9.2
Propanol 2470 3240 4580 4.43 7.71 8.94
Butanol 2340 2830 3240 4.40 7.4 8.7
Propanol
Conc.
5% 1980 2410 3840 5.5 8.2 9.6
8% 2330 3140 4150 4.8 8.0 9.3
10% 2470 3240 4580 4.43 7.71 8.94
12% 2410 3190 4260 3.9 7.3 8.6
15% 2220 2550 3570 3.5 6.7 8.2
Flow rate
(mL/min.)
0.8 1340 1180 2330 6.7 11.5 13.4
1.0 2110 2660 3880 5.3 9.3 10.7
1.2 2470 3240 4580 4.43 7.71 8.94
1.5 2310 2740 3940 3.54 6.2 7.2
1.8 2010 2110 2140 2.9 5.2 6.0
Experimental parameter Selectivity factor (α)
pH   LAM KCZ KCZ IDV
3.0 1.75 1.17
4.0 1.74 1.17
4.5 1.74 1.16
5.0 1.74 1.14
6 1.75 1.15
7.0 1.75 1.15
SDS Conc. 0.05 1.66 1.17
0.07 1.74 1.16
0.1 1.90 1.14
0.12 2.13 1.19
0.15 2.31 1.17
Type of organic modifier Methanol 1.74 1.19
Ethanol 1.76 1.16
Propanol 1.74 1.16
Butanol 1.70 1.18
Propanol Conc. 5% 1.50 1.17
8% 1.70 1.16
10% 1.74 1.16
12% 1.90 1.18
15% 1.91 1.22
Flow rate (mL/min.) 0.8 1.72 1.17
1.0 1.75 1.15
1.2 1.74 1.16
1.5 1.75 1.16
1.8 1.80 1.15
Experimental parameter Resolution factor (Rs)
pH   LAM KCZ KCZ IDV
3.0 3.80 1.30
4.0 3.81 1.30
4.5 3.83 1.33
5.0 3.80 1.32
6 3.81 1.30
7.0 3.81 1.30
SDS Conc. 0.05 4.5 2.1
0.07 3.83 1.33
0.1 3.1 1.26
0.12 2.5 1.16
0.15 2.0 1.0
Type of organic modifier Methanol 2.8 1.11
Ethanol 3.2 1.20
Propanol 3.83 1.33
Butanol 3.9 1.40
Propanol Conc. 5% 5.1 1.8
8% 4.2 1.50
10% 3.83 1.33
12% 3.1 1.28
15% 2.66 1.21
Flow rate (mL/min.) 0.8 5.7 2.0
1.0 4.60 1.60
1.2 3.83 1.33
1.5 3.10 1.1
1.8 2.55 0.9
LAM: Lamivudine
KCZ: Ketoconazole
IDV: Indinavir
Table 1: Effect of different experimental parameters on the column efficiency.