| Experimental parameter |
Number of theoretical plates |
Capacity factor K` |
| LAM |
KCZ |
IDV |
LAM |
KCZ |
IDV |
|
pH |
3.0 |
2360 |
2880 |
4310 |
4.40 |
7.76 |
9.0 |
| 4.0 |
2410 |
3150 |
4470 |
4.41 |
7.75 |
9.0 |
| 4.5 |
2470 |
3240 |
4580 |
4.43 |
7.71 |
8.94 |
| 5.0 |
2435 |
3210 |
4450 |
4.38 |
7.60 |
8.80 |
| 6 |
2360 |
2910 |
4360 |
4.38 |
7.60 |
8.90 |
| 7.0 |
2310 |
2650 |
4280 |
4.40 |
7.58 |
8.90 |
|
SDS Conc. |
0.05 |
2340 |
3170 |
4450 |
5.0 |
8.3 |
9.7 |
| 0.07 |
2470 |
3240 |
4580 |
4.43 |
7.71 |
8.94 |
| 0.1 |
2440 |
3120 |
4420 |
3.85 |
7.3 |
8.3 |
| 0.12 |
2350 |
2920 |
4320 |
3.0 |
6.4 |
7.6 |
| 0.15 |
2240 |
2840 |
4110 |
2.6 |
6.0 |
7.0 |
Type of organic
modifier |
Methanol |
1180 |
1850 |
2280 |
4.6 |
8.0 |
9.5 |
| Ethanol |
2250 |
2650 |
3110 |
4.5 |
7.9 |
9.2 |
| Propanol |
2470 |
3240 |
4580 |
4.43 |
7.71 |
8.94 |
| Butanol |
2340 |
2830 |
3240 |
4.40 |
7.4 |
8.7 |
Propanol
Conc. |
5% |
1980 |
2410 |
3840 |
5.5 |
8.2 |
9.6 |
| 8% |
2330 |
3140 |
4150 |
4.8 |
8.0 |
9.3 |
| 10% |
2470 |
3240 |
4580 |
4.43 |
7.71 |
8.94 |
| 12% |
2410 |
3190 |
4260 |
3.9 |
7.3 |
8.6 |
| 15% |
2220 |
2550 |
3570 |
3.5 |
6.7 |
8.2 |
Flow rate
(mL/min.) |
0.8 |
1340 |
1180 |
2330 |
6.7 |
11.5 |
13.4 |
| 1.0 |
2110 |
2660 |
3880 |
5.3 |
9.3 |
10.7 |
| 1.2 |
2470 |
3240 |
4580 |
4.43 |
7.71 |
8.94 |
| 1.5 |
2310 |
2740 |
3940 |
3.54 |
6.2 |
7.2 |
| 1.8 |
2010 |
2110 |
2140 |
2.9 |
5.2 |
6.0 |
| Experimental parameter |
Selectivity factor (α) |
| pH |
|
LAM |
KCZ |
KCZ |
IDV |
| 3.0 |
1.75 |
1.17 |
| 4.0 |
1.74 |
1.17 |
| 4.5 |
1.74 |
1.16 |
| 5.0 |
1.74 |
1.14 |
| 6 |
1.75 |
1.15 |
| 7.0 |
1.75 |
1.15 |
| SDS Conc. |
0.05 |
1.66 |
1.17 |
| 0.07 |
1.74 |
1.16 |
| 0.1 |
1.90 |
1.14 |
| 0.12 |
2.13 |
1.19 |
| 0.15 |
2.31 |
1.17 |
| Type of organic modifier |
Methanol |
1.74 |
1.19 |
| Ethanol |
1.76 |
1.16 |
| Propanol |
1.74 |
1.16 |
| Butanol |
1.70 |
1.18 |
| Propanol Conc. |
5% |
1.50 |
1.17 |
| 8% |
1.70 |
1.16 |
| 10% |
1.74 |
1.16 |
| 12% |
1.90 |
1.18 |
| 15% |
1.91 |
1.22 |
| Flow rate (mL/min.) |
0.8 |
1.72 |
1.17 |
| 1.0 |
1.75 |
1.15 |
| 1.2 |
1.74 |
1.16 |
| 1.5 |
1.75 |
1.16 |
| 1.8 |
1.80 |
1.15 |
| Experimental parameter |
Resolution factor (Rs) |
| pH |
|
LAM |
KCZ |
KCZ |
IDV |
| 3.0 |
3.80 |
1.30 |
| 4.0 |
3.81 |
1.30 |
| 4.5 |
3.83 |
1.33 |
| 5.0 |
3.80 |
1.32 |
| 6 |
3.81 |
1.30 |
| 7.0 |
3.81 |
1.30 |
| SDS Conc. |
0.05 |
4.5 |
2.1 |
| 0.07 |
3.83 |
1.33 |
| 0.1 |
3.1 |
1.26 |
| 0.12 |
2.5 |
1.16 |
| 0.15 |
2.0 |
1.0 |
| Type of organic modifier |
Methanol |
2.8 |
1.11 |
| Ethanol |
3.2 |
1.20 |
| Propanol |
3.83 |
1.33 |
| Butanol |
3.9 |
1.40 |
| Propanol Conc. |
5% |
5.1 |
1.8 |
| 8% |
4.2 |
1.50 |
| 10% |
3.83 |
1.33 |
| 12% |
3.1 |
1.28 |
| 15% |
2.66 |
1.21 |
| Flow rate (mL/min.) |
0.8 |
5.7 |
2.0 |
| 1.0 |
4.60 |
1.60 |
| 1.2 |
3.83 |
1.33 |
| 1.5 |
3.10 |
1.1 |
| 1.8 |
2.55 |
0.9 |
|
LAM: Lamivudine
KCZ: Ketoconazole
IDV: Indinavir |
| Table 1: Effect of different experimental parameters on the column efficiency. |