Parameters Lamivudine Ketoconazole Indinavir Retention time Theoritical plate Capacity factor Retention time Theoritical plate Capacity factor Retention time Theoritical plate Capacity factor 0.05MSDS 4.2 2340 3.5 6.50 3170 8.3 7.50 4450 9.70 0.07MSDS 3.8 2470 4.43 6.1 3240 7.71 6.9 4580 8.94 0.1 MSDS 3.4 2440 1.5 5.80 3120 7.30 6.5 4420 8.3 8 %propanol 4.1 2330 4.8 6.3 3140 8.0 7.2 4150 9.3 10%propanol 3.8 2470 4.43 6.1 3240 7.71 6.9 4580 8.94 12%propanol 3.4 2410 3.9 5.8 3190 7.30 6.7 4260 8.6 pH 4 3.82 2410 4.45 6.2 3150 7.75 7.0 4470 9.0 pH 4.5 3.8 2470 4.43 6.1 3240 7.71 6.9 4580 8.94 pH 5.0 3.7 2435 4.38 6.0 3210 7.60 6.8 4450 8.8 1.0mL/min 4.4 2110 5.3 7.2 2260 9.3 5.50 3880 10.7 1.2mL/min 3.8 2470 4.43 6.1 3240 7.71 6.9 4580 8.94 1.5mL/min 3.2 2310 3.54 5.0 2740 6.2 5.7 3940 7.2

Table 4: Results of the robustness of the proposed method.