Sample Proposed method Comparison method [3] Amount added, µg/mL Amount found, µg/mL % Recovery Amount added, µg/mL % Recovery 1) Plasma 1 1 100 4 100 5 5.01 100.2 8 100.5 10 9.96 99.6 12 99.14 Mean±SD     99.93±0.31   99.88±0.69 t-test   0.26 (2.132)**     F-test   4.95 (19.00)**                 2) Urine 1 1 100 4 100   5 5.02 100.4 8 99.9   10 9.99 99.9 12 100.7 Mean±SD     100.10±0.26   100.20±0.44 t-test   1.15 (2.132)**     F-test   2.86 (19.00)**

** The value of tabulated t and F, (at p = 0.05) (13)

Table 4: Determination of idrocilamide in biological fluids by the proposed and comparison methods