Method of extraction Total Vitamin B12 content in Spirulina biomass (µg/100g) by microbiological assay Total Vitamin B12 content in Spirulina biomass (µg/100g) by Chemiluminescence assay
Method 1 220±1.8 213±0.4
Method 2 142±0.9 207±1.7
Method 3 116±0.8 65±0.9
Method 4 460±6.2 211±2.2
Method 5 184±1.4 190±0.5
Table 1: Quantification of vitamin B12 by Microbilogical method using Lactobacillus delbrueckii