Constituent Regression equationa Correlation coefficient (r2) Linearity range(μg mL-1) LOD (μg mL-1)
gamabufotalin y = 9213.8x + 14106 0.9994 3.13-200 0.08
arenobufagin y = 10915x + 5079.1 0.9998 1.56-100 0.07
telocinobufagin y = 12147x + 12169 0.9996 2.34-150 0.06
desacetylcinobufotalin y = 11225x + 10293 0.9997 2.34-150 0.07
bufotalin y = 9079.4x + 14123 0.9996 3.75-240 0.09
cinobufotalin y = 10229x + 8819.8 0.9998 2.81-180 0.08
bufalin y = 7979.2x + 13995 0.9996 4.69-300 0.10
cinobufagin y = 10636x + 23504 0.9997 6.25-400 0.07
resibufogenin y = 10860x + 28880 0.9998 7.03-450 0.07
ay: peak area of components; x: concentration of components.
Table 1: Regression equation, linear range and LODs of the developed method.