| Constituent |
Regression equationa |
Correlation coefficient (r2) |
Linearity range(μg mL-1) |
LOD (μg mL-1) |
| gamabufotalin |
y = 9213.8x + 14106 |
0.9994 |
3.13-200 |
0.08 |
| arenobufagin |
y = 10915x + 5079.1 |
0.9998 |
1.56-100 |
0.07 |
| telocinobufagin |
y = 12147x + 12169 |
0.9996 |
2.34-150 |
0.06 |
| desacetylcinobufotalin |
y = 11225x + 10293 |
0.9997 |
2.34-150 |
0.07 |
| bufotalin |
y = 9079.4x + 14123 |
0.9996 |
3.75-240 |
0.09 |
| cinobufotalin |
y = 10229x + 8819.8 |
0.9998 |
2.81-180 |
0.08 |
| bufalin |
y = 7979.2x + 13995 |
0.9996 |
4.69-300 |
0.10 |
| cinobufagin |
y = 10636x + 23504 |
0.9997 |
6.25-400 |
0.07 |
| resibufogenin |
y = 10860x + 28880 |
0.9998 |
7.03-450 |
0.07 |
|
| ay: peak area of components; x: concentration of components. |
| Table 1: Regression equation, linear range and LODs of the developed method. |
