Components Sample contents
(μg)
Add quantity
(μg)
Measured contents
(μg, mean±SD)
Recovery
()
RSD
()
gamabufotalin 409.69 328 743.23±1.00 101.69 0.13
410 818.71±5.72 99.76 0.70
492 892.90±8.53 98.21 0.96
arenobufagin 144.21 116 258.94±1.53 98.91 0.59
145 289.14±3.00 99.95 1.04
174 317.44±0.94 99.56 0.30
telocinobufagin 69.22 56 125.43±0.44 100.38 0.35
70 140.00±0.91 101.12 0.65
84 152.85±1.04 99.56 0.68
desacetylcinobufotalin 410.23 328 737.35±5.53 99.73 0.75
410 813.20±3.22 98.29 0.40
492 901.90±6.34 99.93 0.70
bufotalin 550.17 440 987.98±5.28 99.5 0.53
550 1100.71±9.70 100.1 0.88
660 1213.61±4.45 100.52 0.37
cinobufotalin 128.42 102 229.02±0.85 98.24 0.37
128 257.68±0.81 100.99 0.31
154 279.62±1.82 98.44 0.65
bufalin 1619.28 1296 2912.44±11.29 99.78 0.39
1620 3213.43±10.98 98.4 0.34
1944 3552.52±4.07 99.45 0.11
cinobufagin 710.31 568 1274.41±15.65 99.31 1.23
710 1414.55±3.78 99.19 0.27
852 1557.59±18.10 99.45 1.16
resibufogenin 2332.81 1864 4184.88±11.26 99.36 0.27
2330 4659.78±51.33 99.87 1.10
2796 5115.34±1.45 99.52 0.03
Table 3: Recovery of the developed method.