Surfactant (SDS) w/w % Co-surfactant (butanol) w/w% Oil (octane) w/w % Phosphate buffer w/w% Column temperature (°C) Retention Time Pressure of column weight concentration 4.5 7.6 0.8 87.1 15mM 30 4.82min 171

Table 1: Shows the optimum parameter of mobile phase.