Parameters SS BH ET
Absorption maxima (nm) 275 275 275
Linearity range (µg spot-1) 1-6 1-6 0.5-3.0
Coefficient of determination (r2) 0.998 0.997 0.998
Correlation coefficient (r) 0.999 0.999 0.999
Regression equation (Ya) Y=488.2x+754.7 Y=292.3x+116.7 Y=5291x+3476
Slope (m) 488.2 292.3 5291
tcalm 1.3234 1.2857 0.1323
Confidence intervalm 488.2 ± 21.67 292.3 ± 3.99 5291 ± 100.62
Intercept (c) 754.7 116.7 3476
tcalm 0.5249 0.6368 1.4692
Confidence intervalm 754.7 ± 59.23 116.7 ± 19.62 3476 ± 103.52
Limit of detection LOD, (ng) 100 200 10
Limit of quantification LOQ (ng) 330 660 33
Precision, Intra-day (%RSD)
Inter-day (%RSD)
0.79
0.53
0.94
1.21
0.31
0.26
Table 1:Validation parameters of HPTLC method for SS, BH and ET