| Parameters |
SS |
BH |
ET |
| Absorption maxima (nm) |
275 |
275 |
275 |
| Linearity range (µg spot-1) |
1-6 |
1-6 |
0.5-3.0 |
| Coefficient of determination (r2) |
0.998 |
0.997 |
0.998 |
| Correlation coefficient (r) |
0.999 |
0.999 |
0.999 |
| Regression equation (Ya) |
Y=488.2x+754.7 |
Y=292.3x+116.7 |
Y=5291x+3476 |
| Slope (m) |
488.2 |
292.3 |
5291 |
| tcalm |
1.3234 |
1.2857 |
0.1323 |
| Confidence intervalm |
488.2 ± 21.67 |
292.3 ± 3.99 |
5291 ± 100.62 |
| Intercept (c) |
754.7 |
116.7 |
3476 |
| tcalm |
0.5249 |
0.6368 |
1.4692 |
| Confidence intervalm |
754.7 ± 59.23 |
116.7 ± 19.62 |
3476 ± 103.52 |
| Limit of detection LOD, (ng) |
100 |
200 |
10 |
| Limit of quantification LOQ (ng) |
330 |
660 |
33 |
Precision, Intra-day (%RSD)
Inter-day (%RSD) |
0.79
0.53 |
0.94
1.21 |
0.31
0.26 |