Parameters SS BH ET
Absorption maxima (nm) 275 275 275
Linearity range (µg mL-1) 1-32 1-32 0.5-16
Coefficient of determination (r2) 0.997 0.998 0.998
Correlation coefficient (r) 0.999 0.999 0.999
Regression equation (Ya) Y=4253x-2508 Y=16462x-403.9 Y=25609x-3524
Slope (m) 4253 16462 25609
tcalm 1.5241 1.2872 0.5341
Confidence intervalm 4253 ± 91.67 16462 ± 59.77 25609 ± 274.62
Intercept (c) -2508 -403.9 -3524
tcalm 0.0007 0.0162 0.0100
Confidence intervalm -2508 ± 649.06 -403.9 ± 544.72 -3524 ± 923.37
LOB (µg mL-1) 0.0455 0.0606 0.0045
LOD (µg mL-1) 0.1500 0.2000 0.0150
LOQ (µg mL-1) 0.4950 0.6600 0.0495
Precision, Intra-day (%RSD)
Inter-day (%RSD)
0.72
1.17
0.45
0.89
0.30
0.52
Table 2: Validation parameters for RP-HPLC method of SS, BH and ET.