Nominal Concentration
(µg spot-1)
Amount recovered
( µg spot-1)
%RSD Mean recovery
(%w/w)
tcal CI
SS BH ET SS BH ET SS BH ET SS BH ET SS BH ET SS BH ET
Intra-day (n=3)
1.6 6.4 160 1.58 6.38 159.99 1.2658 0.5041 0.3908 98.75 99.64 99.99 1.7104 1.2526 0.0277  ± 0.0297  ± 0.0478  ± 0.9298
2.0 8.0 200 1.99 7.97 199.49 1.2625 0.5749 1.4835 99.67 99.63 99.75 0.4573 1.1296 0.2958  ± 0.0374  ± 0.0682  ± 4.4012
2.4 9.6 240 2.39 9.59 239.95 1.2800 0.2623 0.6205 99.44 99.93 99.98 0.7517 0.4585 0.0620  ± 0.0454  ± 0.0374  ± 2.2141
Inter-day (n=6)
1.6 6.4 160 1.59 6.38 159.27 1.1933 0.4996 1.3151 99.38 99.71 99.55 1.2832 1.4047 0.8461  ± 0.0199  ± 0.0335  ± 2.2026
2.0 8.0 200 1.99 7.99 199.79 0.9341 0.7405 1.0814 99.67 99.96 99.89 0.8743 0.1379 0.2417  ± 0.0196  ± 0.0623  ± 2.2719
2.4 9.6 240 2.39 9.56 239.49 0.8092 0.6255 0.9163 99.93 99.60 99.79 0.2102 1.5641 1.5641  ± 0.0204  ± 0.0629  ± 2.3076
Table 3: Results of recovery study of SS, BH and ET by HPTLC Method.