Chemical Calibration curve a Correlation coefficient (r2) Linear range (ng/ml) LOD b (ng/ml) LOQ c (ng/ml) Ferulic Acid y=0.3477x + 4.4147   0.9937 2.5-4000 0.5 2.5

a The calibration curve was contructed by plotting the peak area versus each concentration. The calibration curve was derived from three data points, n = 3, and the SD was < 5% of the Mean. b LOD refers to the limits of detection c LOQ refers to the limits of quantification.

Table 1: Calibration curves of ferulic acid standard solution in rat plasma were determined by using UPLC-MS/MS method during method validation.