Ferulic acid
Added (ng/ml)
Precision Repeatability (n = 6) Recovery a(n = 3)
 Intra-day (n=6) b

Inter-day (n=6)c

Mean  (ng/ml) RSD (%) Mean (%) RSD (%)
Mean(ng/ml) RSD (%) Mean(ng/ml) RSD (%)
5 5.31 16.47 5.12 13.31 5.17 12.32 83.6 9.31
500 452.71 2.33 470.44 0.75 488.85 8.67 75.8 1.55
3000 2688.49 3.83 2988.17 3.32 2989.05 8.94 72.5 0.81
Docetaxel (I.S.) (500ng/ml) - - - - - - 88.2 2.14
Ferulic acid at different concentrations of 5, 500, 3000 ng/ml in rat plasma were prepared and then injected into UPLC-MS/MS. Mean of the each concentration (ng/mL) of
ferulic acid in rat plasma, RSD (%) and RE (%) was calculated, n = 3.
a Recovery (%)= 100 X (amount found-original amount)/ amount spiked. The data was presented as average of three independent determinations.
b The intra-day analysis refers to the sample examined for six replicates within one day.
c The inter-day analysis refers to the sample examined in duplicates over three.
Table 3: Precision and accuracy of the UPLC-MS/MS method to determine ferulic acid in rats plasma (n=3 days, six replicates per day).