Table 3

Ferulic acid Added (ng/ml)	Precision				Repeatability (n = 6)		Recovery ^a(n = 3)
	Intra-day (n=6) ^b		Inter-day (n=6)^c		Mean (ng/ml)	RSD (%)	Mean (%)	RSD (%)
	Mean(ng/ml)	RSD (%)	Mean(ng/ml)	RSD (%)	Mean (ng/ml)	RSD (%)	Mean (%)	RSD (%)
5	5.31	16.47	5.12	13.31	5.17	12.32	83.6	9.31
500	452.71	2.33	470.44	0.75	488.85	8.67	75.8	1.55
3000	2688.49	3.83	2988.17	3.32	2989.05	8.94	72.5	0.81
Docetaxel (I.S.) (500ng/ml)	-	-	-	-	-	-	88.2	2.14

Ferulic acid at different concentrations of 5, 500, 3000 ng/ml in rat plasma were prepared and then injected into UPLC-MS/MS. Mean of the each concentration (ng/mL) of
ferulic acid in rat plasma, RSD (%) and RE (%) was calculated, n = 3.
a Recovery (%)= 100 X (amount found-original amount)/ amount spiked. The data was presented as average of three independent determinations.
b The intra-day analysis refers to the sample examined for six replicates within one day.
c The inter-day analysis refers to the sample examined in duplicates over three.

Table 3: Precision and accuracy of the UPLC-MS/MS method to determine ferulic acid in rats plasma (n=3 days, six replicates per day).