Parameters MET DAB
Absorption Wavelength(nm) 666 632
Beers law range (µg/mL) 4-20 1-6
Molar absorptivity (L mol/cm) 0.00486 0.00075
Sandellsensitivitya, (µg/cm) 0.1744 0.0083
Limit of Detection (µg/mL) 0.46 0.0478
Limit of Quantification(µg/mL) 1.158 0.248
Correlation coefficient(r2) 0.999 0.9995
Slope(m) 0.035 0.1037
Intercept(c) 0.246 0.0083
Regression equation(yb) Y = 0.035x + 0.246 Y=0.1037x+0.0083
alimit of determination as the weight in μg per mL of solution which corresponds to an absorbance of A=0.001 measured in a cuvette of cross sectional area 1 cm2 and l=1 cm. yb =a+bx, where y is the absorbance, x is concentration in μg/mL, a is intercept and b is slope.
Table 1: System Suitability Parameters of MET and DAB.