| Parameters |
MET |
DAB |
| Absorption Wavelength(nm) |
666 |
632 |
| Beers law range (µg/mL) |
4-20 |
1-6 |
| Molar absorptivity (L mol/cm) |
0.00486 |
0.00075 |
| Sandellsensitivitya, (µg/cm) |
0.1744 |
0.0083 |
| Limit of Detection (µg/mL) |
0.46 |
0.0478 |
| Limit of Quantification(µg/mL) |
1.158 |
0.248 |
| Correlation coefficient(r2) |
0.999 |
0.9995 |
| Slope(m) |
0.035 |
0.1037 |
| Intercept(c) |
0.246 |
0.0083 |
| Regression equation(yb) |
Y = 0.035x + 0.246 |
Y=0.1037x+0.0083 |
|
| alimit of determination as the weight in μg per mL of solution which corresponds
to an absorbance of A=0.001 measured in a cuvette of cross sectional area 1 cm2
and l=1 cm. yb =a+bx, where y is the absorbance, x is concentration in μg/mL, a
is intercept and b is slope. |
| Table 1: System Suitability Parameters of MET and DAB. |