Figure 4: Morphological changes and oxidative status of glomeruli following chronic drug monotherapy and combination therapy. (A) PAS staining. Scale bar=50 μm. (B) Glomerular size. *p<0.05 vs. Cont. (C) ROS production as measured by DCFH staining in isolated glomeruli. Scale bar=50 μm. (D) Relative DCF intensity in glomeruli. N=20 glomeruli for each treatment group. *p<0.05 vs. Cont. †p<0.05 vs. DM.