Parameters Azithromycin Sparfloxacin Cephalexin monohydrate
λmax(nm) 595 620 600
Molar ratio (D:R) 2:1 2:1 2:1
Linear range (μg mL-1) 0-50 10-80 10-170
Slope 0.0178 0.0096 0.0007
Intercept 0.0418 -0.1028 -0.0080
Sandell’s sensitivity (μg cm-2) 0.0559 0.1034 1.3920
Correlation coefficient 0.9871 0.9967 0.9992
Stability constant (log K) 6.19 ± 0.04 5.00 ± 0.07 4.05 ± 0.05
Molar absorptivity (L mol-1 cm-1) 1.369×104 3.774×103 2.620×102
LODa(μg mL-1 ) 0.10 0.21 1.69
LOQb (μg mL-1 ) 0.35 0.71 5.63
%RSDc(at 10 μg mL-1) 0.55 0.33 3.06
ΔGod(kJ mol-1) -2.725×103 -2.393×103 -1.938×103
aLimit of detection
bLimit of Quantitation
cRelative standard deviation
dFree energy change
Table 1: Qualitative and statistical parameters for proposed methods.