| Method Parameter |
Normal Condition |
Deliberate Change |
%RSD of peak area (n=3) |
| ALP |
MEB |
| Flow rate |
1.0 ml/min |
0.8 ml/min |
0.49 |
1.14 |
| 1.2 ml/min |
0.60 |
1.06 |
| Mobile phase ratio |
Methanol: Buffer (70:30) |
72:28 |
1.01 |
1.90 |
| 68:32 |
1.12 |
1.81 |
| pH of mobile phase ratio |
5.0 |
5.2 |
0.58 |
0.67 |
| 4.8 |
0.69 |
1.02 |
|