Calibration set Validation set
Sample No. Nifedipine concentration
(µg/mL)
Atenolol concentration
(µg/mL)
Nifedipine concentration
(µg/mL)
Atenolol concentration
(µg/mL)
1 5 25 10 25
2 5 20 7.5 22.5
3 15 20 12.5 30
4 7.5 30 10 27.5
5 15 22.5 15 25
6 10 30 12.5 20
7 7.5 25 10 20
8 12.5 22.5 12.5 27.5
9 15 27.5 - -
10 15 30 - -
11 5 30 - -
12 5 27.5 - -
13 12.5 25 - -
14 7.5 27.5 - -
15 5 22.5 - -
16 7.5 20 - -
17 10 22.5 - -
Table 1: The five level two factor experimental design of the calibration and validation set mixtures.