Calibration set
Validation set
Sample No.
Nifedipine concentration
(µg/mL)
Atenolol concentration
(µg/mL)
Nifedipine concentration
(µg/mL)
Atenolol concentration
(µg/mL)
1
5
25
10
25
2
5
20
7.5
22.5
3
15
20
12.5
30
4
7.5
30
10
27.5
5
15
22.5
15
25
6
10
30
12.5
20
7
7.5
25
10
20
8
12.5
22.5
12.5
27.5
9
15
27.5
-
-
10
15
30
-
-
11
5
30
-
-
12
5
27.5
-
-
13
12.5
25
-
-
14
7.5
27.5
-
-
15
5
22.5
-
-
16
7.5
20
-
-
17
10
22.5
-
-
Table 1:
The five level two factor experimental design of the calibration and validation set mixtures.