Parameters Nifedipine Atenolol Reference value *
Resolution (Rs) - 18.32 ≥2
Relative retention time 1.78 4.84 >1
Tailing factor (t) 0.85 1.01 0.5-2
Capacity factor ( K’ ) 1.78 1.70 >2
Height equivalent to theoretical plates (H=L/N) 0.006 0.0008 The smaller the value, the more efficient separation
Number of theoretical plates (N) 2234 10929 The higher the value, the more efficient separation
*FDA regulations [25]
Table 7: System suitability parameters of analysis of nifedipine and atenolol by the proposed HPLC method.