| Theoretical
concentration
(ng/mL) |
1.Run |
Measured concentration (ng/mL) |
|
1.Mean ±SD |
RSD |
Accuracy
(%) |
| Intra-day variation (Six replicates at each concentration) |
| 255 |
1 |
270 ±9.08 |
3.36 |
106 |
| 2 |
278 ±7.23 |
2.60 |
109 |
| 3 |
282 ±14.0 |
4.97 |
110 |
| 4 |
258 ±30.9 |
1.09 |
101 |
| 765 |
1 |
782 ±21.1 |
2.70 |
102 |
| 2 |
778 ±25.3 |
3.25 |
102 |
| 3 |
866 ±34.3 |
3.96 |
113 |
| 4 |
704 ±32.3 |
4.58 |
92.0 |
| 2782 |
1 |
2842 ±142 |
5.00 |
102 |
| 2 |
2787 ±102 |
3.66 |
100 |
| 3 |
3180 ±212 |
6.66 |
114 |
| 4 |
2715 ±171 |
6.31 |
97.6 |
| 4404 |
1 |
4528 ±279 |
6.15 |
103 |
| 2 |
4389 ±204 |
4.65 |
99.7 |
| 3 |
5028 ±399 |
7.93 |
114 |
| 4 |
4309 ±332 |
7.70 |
97.8 |
| Inter-day variation (twenty four replicates at each concentration) |
| 255 |
|
272 ±19.1 |
7.01 |
107 |
| 765 |
782 ±64.5 |
8.24 |
102 |
| 2782 |
2881 ±237 |
8.21 |
104 |
| 4404 |
4563 ±407 |
8.92 |
104 |
|
| RSD: Relative standard deviation (SD x 100/Mean) |
| Table 1: Intra- and inter-day precision of determination of vorinostat in rat plasma. |
