Theoretical concentration (ng/mL)   1.Run Measured concentration (ng/mL)
1.Mean ±SD RSD Accuracy (%)
Intra-day variation (Six replicates at each concentration)
255 1 270 ±9.08 3.36 106
2 278 ±7.23 2.60 109
3 282 ±14.0 4.97 110
4 258 ±30.9 1.09 101
765 1 782 ±21.1 2.70 102
2 778 ±25.3 3.25 102
3 866 ±34.3 3.96 113
4 704 ±32.3 4.58 92.0
2782 1 2842 ±142 5.00 102
2 2787 ±102 3.66 100
3 3180 ±212 6.66 114
4 2715 ±171 6.31  97.6
4404 1 4528 ±279 6.15 103
2 4389 ±204 4.65 99.7
3 5028 ±399 7.93 114
4 4309 ±332 7.70 97.8
Inter-day variation (twenty four replicates at each concentration)
255   272 ±19.1 7.01 107
765 782 ±64.5 8.24 102
2782 2881 ±237 8.21 104
4404 4563 ±407 8.92 104
RSD: Relative standard deviation (SD x 100/Mean)
Table 1: Intra- and inter-day precision of determination of vorinostat in rat plasma.