Freeze and Thaw LQC MQC HQC
Concentration(ng/mL) 50.000 250.000 1000.000
Cycle 1 47.621 243.025 976.789
Cycle 2 42.339 273.978 953.351
Cycle 3 41.120 226.444 941.214
Mean (N = 3) 43.6933 235.8157 957.1180
S.D (+/-) 3.4556 8.4994 18.0842
CV (%) 7.91 3.60 1.89
% Nominal 87.39 94.33 95.71
Short Term Plasma at Room Temperature LQC MQC HQC
 Concentration (ng/mL) 50.000 250.000 1000.000
 After 1 hr 43.674 234.781 961.975
 After 2 hr 45.123 257.336 942.312
 After 3 hr 37.978 223.025 982.167
 Mean (N = 3) 42.2583 238.3807 962.1513
 S.D (+/-) 3.4556 17.43644 19.928.9
 CV (%) 8.49 7.31 2.07
 % Nominal 84.52 95.35 96.22
Long Term Plasma Sample at – 70° C LQC MQC HQC
Nominal Concentration (ng/mL) 50.000 250.000 1000.000
 After 1 Weeks 38.125 241.267 925.139
 After 2 Weeks 46.789 252.325 917.025
 After 4 Weeks 39.781 248.026 943.976
 Mean (N = 3) 41.5650 247.2060 928.7133
 S.D (+/-)  4.59926 5.57442  13.82646
 CV (%)  11.07  2.25  1.49
 % Nominal  83.13  98.88  92.87
Table 3: Stability studies.