Parameter Method I Method II Peak Area (Mean ± %RSD ) 0.6369±1.504 0.6753±1.037 Theoretical plates (EP) (Mean ± %RSD ) 15472.83±0.3301 17800 ±0.3628% Retention time (Mean ± %RSD ) 7.134±0.0289 7.003±0.1474 Linearity (ng mL-1) 0.5 - 5 0.5– 5 Regression equation  0.1389x – 0.0262 0.138x – 0.0025 Correlation Coefficient (R2) 0.9929 0.996 LOD (ng mL-1) 1.643 1.100 LOQ (ng mL-1) 5.026 3.600 *Indicates mean of three replicates, RSD= Relative standard deviation, SE= Standard error

Table 1: Chromatographic characteristics of system suitability, linearity and sensitivity.