Parameter Normal Condition Change Condition Change in % RSD  
Flow Rate 0.4 mL min-1 0.396 mL min-1
0.404 mL min-1
0.1293
0.1281
Method I
0.4 mL min-1 0.396 mL min-1
0.404 mL min-1
1.5127
0.0312
Method II
RT= Retention time, EP = Theoretical plates, RSD= Relative standard deviation, SE= Standard error
Table 5: Robustness of the experiment.