| Factorial Array and their responses at positive & negative [(+) & (-)] spaces |
| Spaces |
Levels |
A |
Variables Unit |
B |
C in terms |
Coded |
| Ratio |
A: B ml |
| Negative |
-1.0 |
4 |
|
6 |
0.5 |
1 : 2 |
1 |
| -0.5 |
5 |
|
5 |
1.0 |
2 : 2 |
2 |
| Zero |
Null |
6 |
ml |
4 |
1.5 |
3 : 2 |
3 |
| Positive |
+0.5 |
7 |
|
3 |
2.0 |
4 : 2 |
4 |
| +1.0 |
8 |
|
2 |
2.5 |
5 : 2 |
5 |
| Designed trails three factor at five level (3*5) array of Smaller is Better level(s) |
| Solutions Analysis No. |
Factorial Levels |
Response |
Recovery (in terms) |
Analytical |
Better to Best |
| Absorbance |
Mean ± SD |
S/NR |
Desirability (1=100%) |
Array |
Finer Fitted |
| A |
B |
C |
Y |
n=3 |
Actual |
Predicted |
Fitness |
Codes |
Levels |
| Isra-01 |
1 |
1 |
1 |
0.233 |
0.263 |
12.653 |
11.698 |
-0.030 |
A1B1C1 |
|
| Isra-02 |
1 |
2 |
2 |
0.252 |
0.268 |
11.972 |
11.520 |
-0.020 |
A1B2C2 |
|
| Isra-03 |
1 |
3 |
3 |
0.276 |
0.283 |
11.182 |
10.987 |
-0.010 |
A1B3C3 |
|
| Isra-04 |
1 |
4 |
4 |
0.291 |
0.270 |
10.722 |
11.378 |
0.980 |
|
A1B4C4 |
| Isra-05 |
1 |
5 |
5 |
0.312 |
0.281 |
10.117 |
11.063 |
0.970 |
|
A1B5C4 |
| Isra-06 |
2 |
1 |
2 |
0.226 |
0.229 |
12.918 |
12.799 |
-0.003 |
A2B1C2 |
|
| Isra-07 |
2 |
2 |
3 |
0.235 |
0.241 |
12.579 |
12.372 |
-0.006 |
A2B2C3 |
|
| Isra-08 |
2 |
3 |
4 |
0.248 |
0.233 |
12.111 |
12.582 |
0.980 |
|
A2B3C4 |
| Isra-09 |
2 |
4 |
5 |
0.247 |
0.265 |
12.146 |
11.597 |
-0.018 |
A2B4C5 |
|
| Isra-10 |
2 |
5 |
1 |
0.241 |
0.229 |
12.36 |
12.763 |
0.988 |
|
A2B5C1 |
| Isra-11 |
3 |
1 |
3 |
0.238 |
0.236 |
12.469 |
12.570 |
0.998 |
|
A3B1C3 |
| Isra-12 |
3 |
2 |
4 |
0.231 |
0.225 |
12.728 |
12.885 |
0.994 |
|
A3B2C4 |
| Isra-13 |
3 |
3 |
5 |
0.247 |
0.261 |
12.146 |
11.719 |
-0.014 |
A3B3C5 |
|
| Isra-14 |
3 |
4 |
1 |
0.241 |
0.246 |
12.360 |
12.214 |
-0.005 |
A3B4C4 |
|
| Isra-15 |
3 |
5 |
2 |
0.238 |
0.228 |
12.469 |
12.783 |
0.990 |
|
A3B5C5 |
| Isra-16 |
4 |
1 |
4 |
0.290 |
0.274 |
10.752 |
11.294 |
0.984 |
|
A4B1C4 |
| Isra-17 |
4 |
2 |
5 |
0.294 |
0.307 |
10.633 |
10.233 |
-0.013 |
A4B2C5 |
|
| Isra-18 |
4 |
3 |
1 |
0.290 |
0.296 |
10.752 |
10.548 |
-0.006 |
A4B3C1 |
|
| Isra-19 |
4 |
4 |
2 |
0.297 |
0.300 |
10.545 |
10.446 |
-0.003 |
A4B4C2 |
|
| Isra-20 |
4 |
5 |
3 |
0.295 |
0.290 |
10.604 |
10.765 |
0.995 |
A4B5C3 |
|
| Isra-21 |
5 |
1 |
5 |
0.333 |
0.320 |
9.5511 |
9.9813 |
-0.4302 |
A5B1C5 |
|
| Isra-22 |
5 |
2 |
1 |
0.335 |
0.306 |
9.4991 |
10.401 |
0.971 |
|
A5B2C1 |
| Isra-23 |
5 |
3 |
2 |
0.325 |
0.314 |
9.7623 |
10.118 |
0.989 |
|
A5B3C2 |
| Isra-24 |
5 |
4 |
3 |
0.330 |
0.325 |
9.6297 |
9.7672 |
-0.030 |
A5B4C3 |
|
| Isra-25 |
5 |
5 |
4 |
0.233 |
0.263 |
12.653 |
11.698 |
-0.010 |
A5B5C4 |
|
| Aristech (*) value indicated the significant variable (optimum) finer as better-fitted level of response; A=Methanol (ml); B=Chloroform; C=Ratio of Methanol (ml): Chloroform (ml); Y=Absorbance at lambda max 327nm of drug concentration (10µg/ml) |
