Factorial Array and their responses at positive & negative [(+) & (-)] spaces
Spaces Levels A Variables Unit B C in terms Coded
Ratio A: B ml
Negative -1.0 4   6 0.5 1 : 2 1
-0.5 5   5 1.0 2 : 2 2
Zero Null 6 ml 4 1.5 3 : 2 3
Positive +0.5 7   3 2.0 4 : 2 4
+1.0 8   2 2.5 5 : 2 5
Designed trails three factor at five level (3*5) array of Smaller is Better level(s)
Solutions Analysis No. Factorial Levels Response Recovery (in terms) Analytical Better to Best
Absorbance Mean ± SD S/NR Desirability (1=100%) Array Finer Fitted
A B C Y n=3 Actual Predicted Fitness Codes Levels
Isra-01 1 1 1 0.233 0.263 12.653 11.698 -0.030 A1B1C1  
Isra-02 1 2 2 0.252 0.268 11.972 11.520 -0.020 A1B2C2  
Isra-03 1 3 3 0.276 0.283 11.182 10.987 -0.010 A1B3C3  
Isra-04 1 4 4 0.291 0.270 10.722 11.378 0.980   A1B4C4
Isra-05 1 5 5 0.312 0.281 10.117 11.063 0.970   A1B5C4
Isra-06 2 1 2 0.226 0.229 12.918 12.799 -0.003 A2B1C2  
Isra-07 2 2 3 0.235 0.241 12.579 12.372 -0.006 A2B2C3  
Isra-08 2 3 4 0.248 0.233 12.111 12.582 0.980   A2B3C4
Isra-09 2 4 5 0.247 0.265 12.146 11.597 -0.018 A2B4C5  
Isra-10 2 5 1 0.241 0.229 12.36 12.763 0.988   A2B5C1
Isra-11 3 1 3 0.238 0.236 12.469 12.570 0.998   A3B1C3
Isra-12 3 2 4 0.231 0.225 12.728 12.885 0.994   A3B2C4
Isra-13 3 3 5 0.247 0.261 12.146 11.719 -0.014 A3B3C5  
Isra-14 3 4 1 0.241 0.246 12.360 12.214 -0.005 A3B4C4  
Isra-15 3 5 2 0.238 0.228 12.469 12.783 0.990   A3B5C5
Isra-16 4 1 4 0.290 0.274 10.752 11.294 0.984   A4B1C4
Isra-17 4 2 5 0.294 0.307 10.633 10.233 -0.013 A4B2C5  
Isra-18 4 3 1 0.290 0.296 10.752 10.548 -0.006 A4B3C1  
Isra-19 4 4 2 0.297 0.300 10.545 10.446 -0.003 A4B4C2  
Isra-20 4 5 3 0.295 0.290 10.604 10.765 0.995 A4B5C3  
Isra-21 5 1 5 0.333 0.320 9.5511 9.9813 -0.4302 A5B1C5  
Isra-22 5 2 1 0.335 0.306 9.4991 10.401 0.971   A5B2C1
Isra-23 5 3 2 0.325 0.314 9.7623 10.118 0.989   A5B3C2
Isra-24 5 4 3 0.330 0.325 9.6297 9.7672 -0.030 A5B4C3  
Isra-25 5 5 4 0.233 0.263 12.653 11.698 -0.010 A5B5C4  
Aristech (*) value indicated the significant variable (optimum) finer as better-fitted level of response; A=Methanol (ml); B=Chloroform; C=Ratio of Methanol (ml): Chloroform (ml); Y=Absorbance at lambda max 327nm of drug concentration (10µg/ml)
Table 1: Designed L25=3*5 Taguchi Array of selected variables and levels via constant drug concentration.