| Finest |
Methanol (A) |
Chloroform (B) |
Ratio (A: B) |
Absorbance (YFST) |
Fitness |
| Desirability (1) |
Error |
| FST-A |
6.10 |
3.00 |
1.00 |
0.2969 |
0.960 |
0.040 |
| FST-B |
6.25 |
3.75 |
1.70 |
0.2910 |
0.860 |
0.150 |
| FST-C |
6.50 |
3.50 |
1.50 |
0.2976 |
0.993 |
0.007 |
| FST-D |
6.75 |
3.00 |
1.50 |
0.2920 |
0.950 |
0.050 |
| FST-E |
7.00 |
3.00 |
1.50 |
0.2910 |
0.870 |
0.130 |
| Quadratic Non-Factorial Response Surface Methodology (RSM) Equation |
| YFST |
Intercept |
A |
B |
C |
AB |
AC |
BC |
A2 |
B2 |
C2 |
| 0.297 |
0.95 |
0.16 |
0.18 |
0.004 |
0.02 |
0.015 |
0.675 |
0.024 |
0.084 |
| Probability |
p<0.01 |
0.01<= p <0.05 |
0.05<= p<0.10 |
p>=0.10 |
| Lambda (λ) max |
327nm |
Finest linearity Range |
2.5-15µg/ml |
| Regression Equation |
y = 0.029x +0.005 |
Recovery (%) |
100.92% |
| Repeatability (%RSD, n = 3) |
0.998 |
Limit of Detection/µg ml-1 |
0.016 |
| Standard solution stability |
|
0.995 |
|
