Group Description Treatment  Number of Mice
1 Normal control Orally administered with 0.1 ml physiological saline 5
2 Diabetic control Orally administered with 0.1 ml physiological saline 5
3 Diabetic reference Orally administered with 0.075 mg glibenclamide (3 mg/kg body weight) in 0.1 ml physiological saline 5
4 Diabetic Orally administered with 1.25 mg extract (50 mg/kg body weight) in 0.1 ml physiological saline 5
5 Diabetic Orally administered with 2.5mg extract (100 mg/kg body weight) in 0.1 ml physiological saline 5
6 Diabetic Orally administered with 5 mg extract (200 mg/kg body weight) in 0.1 ml physiological saline 5
7 Diabetic Orally administered with 7.5mg extract (300 mg/kg body weight) in 0.1 ml physiological saline 5
The experimental mice were randomly divided into seven groups of five animals each. Either 0.1 ml of physiological saline, glibenclamide or the plant extract solution was administered to each experimental mouse orally.
Table 1a: Experimental design for oral administration in mice.