Group Description Treatment Number of Mice
1 Normal control Intraperitoneally administered with 0.1 ml physiological saline 5
2 Diabetic control Intraperitoneally administered with 0.1 ml physiological saline 5
3 Diabetic reference Intraperitoneally administered with 0.025Insulin units (1 IU/kg body weight) in 0.1ml physiological saline 5
4 Diabetic Intraperitoneally administered with 1.25 mg extract (50 mg/kg body weight) in 0.1 ml physiological saline 5
5 Diabetic Intraperitoneally administered with 2.5mg extract (100 mg/kg body weight) in 0.1 ml physiological saline 5
6 Diabetic Intraperitoneally administered with 5 mg extract (200 mg/kg body weight) in 0.1 ml physiological saline 5
7 Diabetic Intraperitoneally administered with 7.5mg extract (300 mg/kg body weight) in 0.1 ml physiological saline 5
The experimental mice were randomly divided into seven groups of five animals each. Either 0.1 ml of physiological saline, insulin, or the plant extract solution wasadministered to each experimental mouse intraperitoneally.
Table 1b: Experimental design for intraperitoneal administration.