Figure 3: Phase-contrast light images of (a-f) hydrogel films and (g-i) commercial PS dish. (a-c) hydrogel films prepared with 4 wt% of LiCl, (d-f) 12 wt% of LiCl. The cell culture time was (a,d,g) 4 h, (b,e,h) 24h and (c,f,i) 48 h. Effect of the LiCl content on (j) projected area, (k) aspect ratio, and (l) length of long axis at cell culture time of 48 h. PS dish was used as control. Bars correspond to the mean ± standard deviation for n= 110 for hydrogel surfaces prepared with different concentration of LiCl.