expression
Figure 4: Effects of OM on NQO1 protein expression in hyperoxia-exposed HPMEC: Human pulmonary microvascular endothelial cells (HPMEC) were treated with DMSO, OM 5, or OM 100 for up to 48 h, following which whole-cell protein was extracted for immunoblotting using anti-NQO1 or β-actin antibodies (A). Densitometric analyses wherein NQO1 band intensities were quantified and normalized to β-actin (B). Values are presented as means ± SEM (n=3). Two-way ANOVA showed an effect of hyperoxia and OM 100 and an interaction between them for the dependent variable, NQO1 protein levels, in this figure. Significant differences between air- and hyperoxia-exposed cells are indicated by *, p<0.05. Significant differences between hyperoxia-exposed DMSO-, OM 5-, and OM 100–treated cells are indicated by †, p<0.05. Significant differences between air-exposed DMSO-, OM 5-, and OM 100–treated cells are indicated by π, p<0.05.