Figure 2: Biacore sensograms illustrating the attachment of HSP60 to CM5 chip via HSP60-specific antibody. (A) The immobilization of the antibody 1 to the CM5 chip surface. The chip was equilibrated in deionized water at a rate of 10 μl /min. The sensogram demonstrates (1) the injection of a mixture of EDC/NHS, (2) the injection of the antibody, and (3) the deactivation of the unreacted sucinimide esters with a basic solution of ethanolamine. The antibody was prepared in a 10 mM sodium acetate buffer (pH 5) at a concentration of 0.1 mg/ml. After the injection of ethanolamine, the chip was re-equilibrated in 10 mM Hepes buffer (pH 7.4) for 24 hours. (B) The binding of HSP60 to the antibody immobilized on the CM5. A 0.580 μM solution of HSP60 in 10 mM hepes buffer (pH 7.4) was injected at a rate of 10 μl /min for 8 min. 500 RU of the protein was attached.