CypD
Figure 3: The binding of CypD to HSP60. A solution of CypD in 10 mM Hepes buffer (pH 7.4) was passed over sensor chips on which HSP60 was immobilized. Retention of the protein on the sensor chip was indicated by a change in RU over the course of the 300 s injection interval. (A) Grey line – a sensogram illustrating the interactions between CypD and HSP60 attached to antibody 1 (residues 300-360), black line – binding of CypD to HSP60 attached to antibody 2 (residues 70-150); (B) binding of CypD to HSP60 attached to antibody 3 (residues 50-100); (C) binding of CypD to HSP60 attached to antibody 4 (residues 250-300); (D) binding of CypD to HSP60 attached to antibody 5 (around a residue 547). The concentration of the injected solution of CypD was 280 nM, and the injection time was 5 min.