Biacore
Figure 4: Biacore sensogram depicting the interactions between HSP60 andCypD. HSP60 is immobilized on the chip surface via antibody 1. The grey lineshows the RU signal as the solution of CypD passes over the chip. The blacklines represent the fitting curves of the association and dissociation parts ofthe sensogram. The kd value is 4.57×10-4 s-1, and the ka value is 3.44×104M-1s-1, as determined by fitting Eqns 1 and 2.