Antibody 1 Antibody 2 Antibody 3 Antibody 4 Antibody 5
Exp. 1 6.39×10-4 ± 3.06×10-5 6.96×10-4 ± 1.57×10-5 1.96×10-3 ± 1.11×10-4 9.98×10-4 ± 6.78×10-5 9.36×10-4 ± 2.15×10-5
Exp. 2 4.57×10-4 ± 6.97×10-6 7.27×10-4 ± 9.89×10-6 1.83×10-3 ± 7.99×10-5 5.51×10-4 ± 2.20×10-5 6.31×10-4 ± 8.94×10-6
Exp. 3 5.54×10-4 ± 2.38×10-5 9.84×10-4 ± 2.00×10-5 1.00×10-3 ± 4.34×10-5 6.54×10-4 ± 9.73×10-6 3.78×10-4 ± 3.27×10-6
Avg 5.50×10-4 ± 1.31×10-5 8.02×10-4 ± 9.09×10-6 16.0×10-4 ± 4.78×10-5 7.34×10-4 ± 2.40×10-5 6.48×10-4 ± 7.84×10-6
Table 2: The values of the dissociation constants, kd, s-1, of HSP60-CypD interactions measured with different orientations of HSP60 immobilized on the antibodies.