Antibody 1 Antibody 2 Antibody 3 Antibody 4 Antibody 5
Exp. 1 1.92×10-8 ±9.48×10-10 2.30×10-8 ±6.07×10-10 4.14×10-8 ± 3.07×10-9 1.63×10-8 ± 1.15×10-9 2.04×10-8 ±5.78×10-10
Exp. 2 1.33×10-8 ±3.16×10-10 3.09×10-8 ±5.64×10-10 6.63×10-8 ± 3.70×10-9 1.24×10-8 ±5.16×10-10 2.40×10-8 ±4.65×10-10
Exp. 3 2.11×10-8 ±9.45×10-10 3.83×10-8 ± 1.04×10-9 2.27×10-8 ± 1.09×10-9 1.88×10-8 ±3.36×10-10 1.27×10-8 ±1.47×10-10
Avg 1.79×10-8 ±4.58×10-10 3.07×10-8 ±4.44×10-10 4.35×10-8 ± 2.46×10-9 1.58×10-8 ±4.36×10-10 1.90×10-8 ±2.52×10-10
Table 3: The values of the dissociation rate constants, KD, M, of HSP60-CypD interactions measured with different orientations of HSP60 immobilized on the antibodies.