Parameters Variables Mean Retention time % RSD Mean peak area %RSD
Flow rate 0.8 ml 6.09 0.123 211.4 0.250
1.2 ml 4.03 0.186 210.8 0.383
Wavelength 237 nm 4.79 0.215 216.0 0.096
241 nm 4.8 0.204 215.6 0.301
Table 5: Result of robustness study.