Days Retention Time Peak area Day-1* 2.557 180230 Day -2* 2.557 181075 Day -3* 2.554 181910 Average 2.557 181071 Standard Deviation 0.002 840.0 % RSD 0.06 0.46

Table 4: Inter-day precision of the proposed method for Hydrochlorothiazide in plasma (on three consecutive days n = 6).