patterns
Figure 2: Agarose gel electrophoresis patterns of pBR322 plasmid DNA (300 ng) cleaved by complex 8 (1.0-7.0 μM), after 1 h incubation time (concentration dependent) Lane 1: control; Lane 2: 1.0 μM 1 + DNA; Lane 3: 2.0 μM 8 + DNA; Lane 4: 3.0 μM 8 + DNA. Lane 5: 4.0 μM 8 + DNA; Lane 6: 5.0 μM 8 + DNA, Lane 7: 6.0 μM 8 + DNA, Lane 8: 7.0 μM 8 + DNA in buffer (5 mM Tris-HCl/50 mM NaCl, pH 7.2 at 25°C).