Parameters Valuesa, SD b, % RSDc
System Suitability         -
RT 4.82 ± 0.02, 0.48
No. of Theoretical Plates 55316 ± 842.61, 1.52
Tailing Factor 0.444 ± 0.01, 1.48
 Peak purity 1.00 ± 0.00, 0.00
Capacity factor 22.44 ± 0.30, 1.32
Linearity 5-25 µgmL-1
Regression equation Y = 20313x + 1687
Correlation coefficient 0.999
Accuracy 99.88% ± 0.63, 0.63
Precision   -
Repeatability 100.04% ± 0.10, 0.10
Intermediate precision  
Day-to-Day 99.99% ± 0.14, 0.14
Analyst-to-analyst 99.93% ± 0.10, 0.10
Response ratio 20523.83 ± 322.68, 1.57
LOQ 0.206 μg mL-1 ± 0.00, 0.22
LOD 0.062 μg mx ± 0.00, 0.22
Robustness   -
Formic acid (0.1%) in aqueous content of mobile phase 99.89% ± 0.21, 0.21
Temperature (± 5%) 99.97% ± 0.11, 0.11
Specificity Ascertained by analyzing standard drug and forced degraded samples
a = mean of six replicates; b = standard deviation; c = percent relative standard deviation
Table 2: Validation parameters for AMD.