| Parameters | 
        Observed value | 
        Recommended value | 
       
      
        | Retention Time (Rt)*  | 
        7.43 ±    0.09 | 
          | 
       
      
        | Number of theoretical plate*  | 
        3124.22 ±    23.25 | 
        > 2000 | 
       
      
        | Tailing factor*  | 
        0.91 ±    0.03 | 
        ≤ 2 | 
       
      
        | Capacity factor | 
        6.43 | 
        > 2 | 
       
      
        | Resolution | 
        7.56 | 
        > 2 | 
       
      
        | %RSD
          (for Retention time) | 
        0.92 | 
        ≤ 1 | 
       
     
    *Each value is the mean recovery ± SD of five determinations |