Table 1

Kinetics Models	Initial parameter values
Michaelis-Menten	V_max = intercept of linear plot*	K_m = slope of linear plot*
Substrate inhibition	V_max = max (v)	K_m = 0.2 × max ([S])	Ks = 3 × K_m
Sigmoidal Hill equation	V_max = max (v)	K_m = 0.2 × max ([S])	n = 1
Sigmoidal two sites model	V_max1 = 0.1 × V_max2	V_max2 = max (v)	K_m1 = 0.2 ×max ([S])	K_m2 = 10 × K_m1
Biphasic two sites model	V_max1 = 0.1 × V_max2	V_max2 = max (v)	K_m1 = 0.2 ×max ([S])	K_m2 = 10 × K_m1
Biphasic linear portion model	V_max1 = max (v)	K_m1 = 0.2 × max ([S])	CLint = V_max1/K_m1
Bisubstrate compulsory ordered	V_max = max (v)	K_mB = 0.2 × max ([B])	K_AX = 0.2 ×max ([AX])	K_mAX = K_AX
Competitive inhibition	V_max = max (v)	K_m = 0.2 × max ([S])	K_i = 3 × K_m
Noncompetitive inhibition	V_max = max (v)	K_m = 0.2 × max ([S])	K_i = 3 × K_m
Uncompetitive inhibition	V_max = max (v)	K_m = 0.2 × max ([S])	K_i = 3 × K_m
Mixed-type inhibition	V_max = max (v)	K_m = 0.2 × max ([S])	K_i = 3 × K_m	Alpha (a) = 1

* Eadie-Hofstee plot; v, observed initial rates; [S], substrate concentrations; [B], aglycone substrate concentrations; [AX], cofactor concentrations.

Table 1: Schemes for auto-estimating initial parameter values in XL_Kinetics.