Hill equation Parameters XL_Kinetics Graphpad Prism SigmaPlot K’ 0.5730 ± 0.0334 0.5730 ± 0.0334 0.5730 ± 0.0334 Vmax 4.8048 ± 0.0610 4.8048 ± 0.0610 4.8048 ± 0.0610 n 1.6801 ± 0.0566 1.6801 ± 0.0566 1.6801 ± 0.0566 Diagnostics       R2 0.9995 0.9995 0.9995 SS 0.0116 0.0116 0.0116 SE 0.0482 0.0482 0.0482 AIC* -29.6357     SC* -29.3974     *AIC and SC are not provided in Graphpad Prism and SigmaPlot.

Table 3: Detailed comparison of XL_Kinetics results with those of Graphpad Prism 5.0 and SigmaPlot 11.0 (Sigmoidal-Hill equation).