Added concentration Repeated reproducibility
(ng/mL)   1st 2nd 3rd  
2 determined 1.93 1.78 2.31  
  amount   1.64 2.16 1.71
    1.99 1.92 1.32  
    1.44 1.77 1.87  
    1.40 1.73 1.94  
  average   1.69 1.87 1.83
  recovery rate (%)   84.0 93.6 93.5
  precision (%) 16.2 9.4 15.7  
40 determined 36.18 33.69 36.58    
  amount   36.77 35.70 34.66
    34.73 34.20 36.32  
    33.41 34.27 35.37  
    32.93 35.47 41.33  
  average   34.8 34.7 36.9
  recovery rate (%)   87.0 86.7 92.1
  precision (%) 4.8 2.5 7.1  
80 determined 69.10 70.07 68.24    
  amount   67.74 71.67 70.69
    67.92 71.34 70.31  
    68.43 68.39 69.47  
    67.79 68.06 76.21  
  average   68.2 69.9 71.0
  recovery rate (%)   85.2 87.4 88.7
  precision (%) 0.8 2.4 4.3  
average of recovery rate from total concentration (%) 88.7 (n=45)
SD of recovery rate from total concentration 7.9 (n=45)
SD: standard deviation
Table 2: Recovery rate of ameziniummetilsulfate (AM) when confirming reproducibility