Figure 1: Time–kill kinetics of a selected strongly-biofilm forming P. aeruginosa isolate against a) amikacine, b) levofloxacin, c) tetracycline, d) tetracycline-loaded chitosan microspheres by coacervation and e) tetracycline-loaded chitosan microspheres by the w/o emulsion methods at 0.5x, 1x, 2x and 4x MIC. The dashed line represents 3 log reductions. ♦, antibiotic-free control; ▀, antibiotic at 4x MIC; ▲, antibiotic at 2x MIC; x, antibiotic at MIC, x, antibiotic at MIC/2, CFU, colony-forming units.