Items Membrane Chromatography Adsorber (70 ml) Large Scale Packed Bed Chromatography (40 L)
Pre-equilibration NA 3 CV = 120 L
Equilibration 10 DV =3.5 L 5 CV = 200 L
Wash 30 DV = 10.5 L 3 CV= 120 L
Regeneration NA 3 CV = 120 L
Storage NA 3 CV = 120 L
Total 14l 680 L
Table 1: Comparison of buffer usage for large scale packed bed chromatography and membrane. Chromatography Adsorber.