Degradation Studies Time Assay of HBB % of peak  area Remarks
1-Blank
2-Photolytic Condition
3-Base hydrolysis (0.1 N NaOH  at 80°C) 
4- Base hydrolysis (5 N NaOH  at 80°C)
5-Acid hydrolysis (0.1 N HCl   at 80°C)
6- Acid hydrolysis ( 5 N HCl   at 80°C)
7-Oxidation by 3 %H2O2  at 25°C
8- Oxidation by 30 %H2O2  at 25°C

12 h
12 h
3 h
12 h
3 h
12 h
3 h
99.00
99.02
25.00
0.00
99.00
65.00
90.00
72.00
No significant
Degradation observed
Degradation products I and II observed
Degradation products I, II and III  observed.
No significant
Degradation observed
Degradation product  II
Observed
Unidentified Degradation products  observed
Degradation product IV
Observed
Table 2: Mass balance for HBB drug substance in presence of degradation products formed during forced degradation studies.