Parameters HBB
Range (μg.mL-1 ) 2-50 (μg.mL-1)
Slope 2.507
Intercept -0.409
Correlation coefficient (r) 0.9997
Accuracy (mean ± SD) 100.20 ± 0.806
(RSD)a* 0.693
(RSD)b* 0.716
(RSD)a*, (RSD)b* the intra-day and inter-day relative standard deviations of the average of concentrations (20, 40 and 50 μg.mL-1 for each )
Table 3: Results of assay validation parameters of HPLC for the determination of HBB.