Taken(μg mL-1) Found*(μg mL-1) Recovery
2.00
10.00
20.00
30.00
40.00
50.00
2.03
10.09
19.95
29.92
39.80
49.90
101.50
100.90
99.75
99.73
99.50
99.80
Mean ± SD 100.20 ± 0.806
*Average of three determinations
Table 4: Results of accuracy for determination of pure authentic of HBB by the proposed HPLC method.