Parameters
Obtained value for HBB
Reference value
Resolution (R
s
)
4.11
>1.5
Capacity factor(K’)
1.48
1- 10 acceptable
Tailing factor (T)
1.20
< 1.5-2
Number of Theoretical plate(N)
5082975
Increases with increases efficiency
HETP (cm.plate
-1
) Height equivalent to theoretical plate
0.4918
The smaller the value, the higher the efficiency
Table 5:
Statistical analysis of parameters required for system suitability testing of HPLC method.