Parameters Obtained value for HBB Reference value
Resolution (Rs) 4.11   >1.5
Capacity factor(K’) 1.48 1- 10 acceptable
Tailing factor (T) 1.20 < 1.5-2
Number of Theoretical plate(N) 5082975 Increases with increases efficiency
HETP (cm.plate-1) Height equivalent to theoretical plate 0.4918 The smaller the value, the higher the efficiency
Table 5: Statistical analysis of parameters required for system suitability testing of HPLC method.