Figure 5: Quantification of p-Akt and Bax protein expressions in sperm cells of three groups. A. The western blot analysis results showing relative expression of p-Akt and Bax protein in non-smoker, tobacco smoke addicted and cannabis smoke addicted subjects. Beta-actin expressions confirmed equal distribution of proteins. B. The graphical representation of densitometric analysis showing highest percentage of both p-Akt (1.08 ± 0.090) and Bax (1.27 ± 0.29) protein expressions in cannabis smoker subjects compared to the other two groups [non-smokers, p-Akt (0.645 ± 0.01), Bax (0.845 ± 0.052) and tobacco smokers, p-Akt (0.86 ± 0.060), Bax (1.01 ± 0.02)].