Figure 1: The amino acid sequence of HIV-1 Nef and mutants. (A) HIVwt sequence (1-79aa) showing domains important for Nef-vesicle induced secretion (BAA-2, P25, 29GVG31, T44, PACS motif, and SMR domain). (B) The HIVdsNef mutant contains two point mutations creating two premature termination codons, one 3’ of each initiator ATGs. (C) The HIVNefsmr5a mutant contains five alanine substitutions in the Secretion Modification Region (66-70aa).